تفاصيل الوثيقة
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نوع الوثيقة |
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مقال في مجلة دورية |
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عنوان الوثيقة |
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A Comparison and Integration of MiSeq and MinION Platforms for Sequencing Single Source and Mixed Mitochondrial Genomes A Comparison and Integration of MiSeq and MinION Platforms for Sequencing Single Source and Mixed Mitochondrial Genomes |
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لغة الوثيقة |
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الانجليزية |
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المستخلص |
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Single source and multiple donor (mixed) samples of human mitochondrial DNA were analyzed
and compared using the MinION and the MiSeq platforms. A generalized variant
detection strategy was employed to provide a cursory framework for evaluating the reliability
and accuracy of mitochondrial sequences produced by the MinION. The feasibility of longread
phasing was investigated to establish its efficacy in quantitatively distinguishing and
deconvolving individuals in a mixture. Finally, a proof-of-concept was demonstrated by integrating
both platforms in a hybrid assembly that leverages solely mixture data to accurately
reconstruct full mitochondrial genomes. |
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ردمد |
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1932-6203 |
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اسم الدورية |
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PloS one |
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المجلد |
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11 |
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العدد |
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12 |
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سنة النشر |
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1437 هـ
2016 م |
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نوع المقالة |
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مقالة علمية |
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تاريخ الاضافة على الموقع |
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Tuesday, July 18, 2017 |
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الباحثون
| Michael R. Lindberg | Lindberg, Michael R. | باحث | دكتوراه | |
| Sarah E Schmedes | Schmedes, Sarah E | باحث | دكتوراه | |
| F Curtis Hewitt | Hewitt, F Curtis | باحث | دكتوراه | |
| Jamie L. Haas | Haas, Jamie L. | باحث | دكتوراه | |
| Krista L. Ternus | Ternus, Krista L | باحث | دكتوراه | |
| Dana R. Kadavy | Kadavy, Dana R | باحث | دكتوراه | |
| Bruce Budowle | Budowle, Bruce | باحث | دكتوراه | |
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الرجوع إلى صفحة الأبحاث
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